![]() ![]() Oral intake of commercially available CP is reported to have physiological effects such as promoting skin pressure ulcer recovery 6, stimulating bone formation and inhibiting bone resorption 7, inhibiting arthritis 8, increasing fat free body mass and grip strength 9, and preventing atherosclerosis 10. These collagen peptides are efficiently absorbed in the digestive tract, appearing in the blood within a few hours of the ingestion of collagen-containing substances 5. Among the metabolites produced during this process are bioactive collagen peptides (CP) resulting from its enzymatic degradation 3, 4. Collagen homeostasis in tissues is maintained by collagen turnover 2. Thus, ACOP may be useful as a new biomarker for collagen metabolism.Ĭollagen, which accounts for approximately 30% of the total protein in animals, is an important component of the extracellular matrix that is essential for cell adhesion and function 1. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Physical activity significantly decreased the urinary ACOP level. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. ![]() We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. Active collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recently-established ELISA. ![]()
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